Arbovirus IgM

Brief Description

Focus Technologies Arbovirus IgM indirect Immunofluorescent assay (IFA) is intended for the qualitative detection of human IgM antibodies to Eastern Equine Encephalitis (EEE), St. Louis encephalitis (SLE), Western Equine Encephalitis (WEE), and California Encephalitis (CE) group viruses. A single sample is used or to identify sero-conversion in acute and convalescent specimens and as an aid in the diagnosis of current or past infection.

Laboratory Unit

Immunology

Methodology

IFA

Specimen Collection

Serum:

Collect in gold top SST tube, centrifuge

Collect in tiger top SST tube, centrifuge

Storage/Transport

Collect blood samples aseptically using approved venipuncture techniques by qualified personnel. Allow blood samples to clot at room temperature prior to centrifugation. Aseptically transfer serum to a tightly closing sterile container (SST).

Serum separated from the clot can be maintained at refrigerated temperature (2-8°C) during transit for no more than five days after collection.  

Serum separated from the clot should be stored frozen (-20°C or colder) and shipped with dry ice.

Acceptable Specimen Type(s)

Serum

Test Request

Possible Results

  • Positive
  • Negative
  • Unsatisfactory

Reference Range

Negative

Unacceptable Conditions

  • Missing testing requisition
  • Missing minimum of 2 patient identifiers
  • Improper labeling
  • Improper storage/transport of specimen
  • No specimen received (empty collection tube).
  • Wrong specimen type
  • Specimens damaged, broken, or leaking upon arrival
  • Grossly hemolyzed or lipemic samples
  • Samples containing particulate matter
  • Samples exhibiting obvious microbial contamination
  • Plasma Samples
  • Received refrigerated more than 5 days after collection

Interfering Substances

  • Specimen collected within 2 weeks after onset of symptoms
  • Previous exposure to or infection with dengue or yellow fever vaccination.
  • Rheumatoid Factor (RF)
  • Cross-reactivity with other mosquito-borne viruses

Result Reported

7-14 business days

Fees

Download this pdf file. Fee schedule

LOINC/Order Codes

40584-5/ (Order code: 1600)