Francisella tularensis Isolates

Brief Description

This real time Polymerase Chain Reaction (RT-PCR) molecular assay is intended for the qualitative detection of Francisella tularensis DNA from culture isolates exhibiting colony morphology and bio-chemical testing consistent with an agent of bioterrorism. Additional reflex testing at the GPHL may be used to confirm specific agents.

Laboratory Unit

Molecular Biology

Methodology

RT-PCR

Specimen Collection

Culture isolates:

Submit a plate or slant exhibiting colony morphology and biochemical testing consistent with Francisella tularensis on BAP agar, CHOC agar or TSA slant.

Storage/Transport

Consultation with district epidemiologist required prior to submission.

Cultures can be maintained at room temperature (15-30°C) during transit.

• FRM-QA 4 Refrigeration Outfit Guide

Acceptable Specimen Type(s)

Culture Isolates

Test Request

• GPHL Apollo Web Portal
• Web Portal Instructions
• For manual orders, download the GPHL Submission Form

Possible Results

• Detected
• Not detected
• Unsatisfactory
• Inconclusive

Reference Range

Not detected

Unacceptable Conditions

• Missing testing requisition form
• Missing minimum of 2 patient identifiers
• Improper labeling
• Specimen damaged, broken, or leaked in transit
• Improper storage/transport of specimen
• No specimen received (empty collection tube).
• Wrong specimen type
• Low volume

Interfering Substances

N/A

Result Reported

1-2 business days

Turnaround times may be affected by the scale of the event.

Fees

No charge

LOINC/Order Codes

33677-6 (Order code: BTC04005 culture)

Additional Information

This test will only be used in response to a biothreat event.  Requestor must contact GPHL at 1-866-PUB-HLTH (1-866-782-4584) prior to submitting specimens for testing.