Francisella tularensis Isolates

Brief Description

This real time Polymerase Chain Reaction (RT-PCR) molecular assay is intended for the qualitative detection of Francisella tularensis DNA from culture isolates exhibiting colony morphology and bio-chemical testing consistent with an agent of bioterrorism. Additional reflex testing at the GPHL may be used to confirm specific agents.

Laboratory Unit

Molecular Biology



Specimen Collection

Culture isolates:

Submit a plate or slant exhibiting colony morphology and biochemical testing consistent with Francisella tularensis on BAP agar, CHOC agar or TSA slant.


Consultation with district epidemiologist required prior to submission.

Cultures can be maintained at room temperature (15-30°C) during transit.

Acceptable Specimen Type(s)

Culture Isolates

Test Request

Possible Results

  • Detected
  • Not detected
  • Unsatisfactory
  • Inconclusive

Reference Range

Not detected

Unacceptable Conditions

  • Missing testing requisition form
  • Missing minimum of 2 patient identifiers
  • Improper labeling
  • Specimen damaged, broken, or leaked in transit
  • Improper storage/transport of specimen
  • No specimen received (empty collection tube).
  • Wrong specimen type
  • Low volume

Interfering Substances


Result Reported

1-2 business days

Turnaround times may be affected by the scale of the event.


No charge

LOINC/Order Codes

33677-6 (Order code: BTC04005 culture)

Additional Information

This test will only be used in response to a biothreat event.  Requestor must contact GPHL at 1-866-PUB-HLTH (1-866-782-4584) prior to submitting specimens for testing.