Hepatitis B antibody (quantitative)

Brief Description

Hepatitis B surface antibody assay is a chemiluminescence immunoassay (CLIA) for the quantitative determination of antibody to hepatitis B surface antigen (anti-HBs) in adult serum. It is intended for quantitative measurement of antibody response following hepatitis B virus (HBV) vaccination, determination of HBV immune status, and for the laboratory diagnosis of HBV disease associated with HBV infection when used in conjunction with other laboratory results and clinical information.

Laboratory Unit



Chemiluminescence Immunoassay (CLIA)

Specimen Collection


Collect in gold top SST tube, centrifuge

Collect in tiger top SST tube, centrifuge


Serum separated from the clot can be maintained at refrigerated temperature (2-8°C) during transit for no more than seven days after collection.  Serum separated from the clot can be aliquoted, stored frozen (-20°C or colder) and shipped with dry ice.

Acceptable Specimen Type(s)


Test Request

Possible Results

  • Reactive >11.0 IU/mL
  • Nonreactive <9.0 IU/mL
  • Unsatisfactory
  • Equivocal 9.0<x<11.0 IU/mL

Reference Range

Nonreactive <9.0 IU/mL

Unacceptable Conditions

  • Missing testing requisition
  • Missing minimum of 2 patient identifiers
  • Improper labeling
  • Improper storage/transport of specimen
  • No specimen received (empty collection tube).
  • Wrong specimen type
  • Low volume
  • Specimen damaged, broken, or leaking upon arrival
  • Specimen grossly hemolyzed, lipemic, turbid, or contaminated
  • Plasma Samples
  • Pooled and heat activated samples
  • Samples with microbial contamination
  • Received refrigerated specimen over 4 days old

Interfering Substances

Fibrin, red blood cells, hemolysis, lipemia, turbidity, particulate matter, erythrocyte debris, bacterial contamination or heat inactivation of the specimens

Result Reported

3-5 business days


No charge

LOINC/Order Codes

5193-8 (Order code: 1635)