Hepatitis B (Prenatal)

Brief Description

The detection of HBsAg in human serum indicates an infection with the Hepatitis B virus (HBV). In acute hepatitis B infection, the first immunological marker to appear is HBsAg which may be present some days or weeks before clinical symptoms begin to appear. HBsAg can be found in pregnant patients with both acute and chronic hepatitis B infections. 

The LIAISON® XL Murex HBsAg Qual assay is an in vitro chemiluminescent immunoassay for the qualitative detection of hepatitis B surface antigen (HBsAg) in human adult and pediatric (2 to 21 years) serum on the LIAISON® XL Analyzer. Assay results in conjunction with other hepatitis B virus (HBV) serological and clinical information, may be used as an aid in the diagnosis of HBV infection in patients with symptoms of hepatitis or who may be at risk for HBV infection. The assay may also be used to screen for HBV infection in pregnant women to identify neonates who are at risk for acquiring hepatitis B during the perinatal period. This assay is not approved for use in screening blood, plasma or tissue donors.

Laboratory Unit

Immunology

Methodology

Chemiluminescence Immunoassay (CLIA)

Specimen Collection

Serum:

Collect in gold top SST tube, centrifuge

Collect in tiger top SST tube, centrifuge

Storage/Transport

Serum separated from the clot can be maintained at refrigerated temperature (2-8°C) during transit for no more than seven days after collection.  Serum separated from the clot can be aliquoted, stored frozen (-20°C or colder) and shipped with dry ice.

• FRM-QA 4 Refrigeration Outfit Guide

Acceptable Specimen Type(s)

Serum

Test Request

• GPHL Apollo Web Portal
• Web Portal Instructions
• For manual orders, download the GPHL Submission Form

Possible Results

• Reactive
• Nonreactive
• Unsatisfactory

Reference Range

Nonreactive

Unacceptable Conditions

• Missing testing requisition
• Missing minimum of 2 patient identifiers
• Improper labeling
• Improper storage/transport of specimen
• No specimen received (empty collection tube).
• Wrong specimen type
• Low volume
• Specimen damaged, broken, or leaking upon arrival
• Specimen grossly hemolyzed, lipemic, turbid, or contaminated
• Plasma Samples
• Received refrigerated specimen over 4 days old

Interfering Substances

Hemolysis, lipemia, turbidity, microbial contamination, particulate matter or erythrocyte debris

Result Reported

3-5 business days

5-7 business days – Antigen positives

Fees

Fee Schedule

LOINC/Order Codes

63557-3 (Order code: 1411)