Hepatitis B (Routine Screen)

Brief Description

Detection of Hepatitis B which is present during acute infections and persists in chronic infections. Determination of carrier status appears at the onset of illness and can persist indefinitely. Presence indicates seroconversion from HBV infection can be used to monitor post vaccination.

Hepatitis B surface antibody assay is a chemiluminescence immunoassay (CLIA) for the quantitative determination of antibody to hepatitis B surface antigen (anti-HBs) in adult serum.

The LIAISON® XL Anti-HBc assay is an in vitro chemiluminescent immunoassay (CLIA) for the qualitative detection of IgG and IgM (total) antibodies to hepatitis B core antigen (anti-HBc) in human serum on the LIAISON® XL Analyzer. Assay results in conjunction with other laboratory results and clinical information may be used as an aid in the diagnosis of hepatitis B virus (HBV) infection in patients with symptoms of hepatitis or who may be at risk for HBV infection.

The LIAISON® XL HBsAg Qual assay is an in vitro chemiluminescent immunoassay for the qualitative detection of hepatitis B surface antigen (HBsAg) in human adult and pediatric (2 to 21 years) serum on the LIAISON® XL Analyzer. Assay results in conjunction with other hepatitis B virus (HBV) serological and clinical information, may be used as an aid in the diagnosis of HBV infection in patients with symptoms of hepatitis or who may be at risk for HBV infection.

Laboratory Unit

Immunology

Methodology

Chemiluminescence Immunoassay (CLIA)

Specimen Collection

Serum:

Collect in gold top SST tube, centrifuge

Collect in tiger top SST tube, centrifuge

Storage/Transport

Serum separated from the clot can be maintained at refrigerated temperature (2-8°C) during transit for no more than four days after collection. Serum separated from the clot can be aliquoted, stored frozen (-20°C or colder) and shipped with dry ice.

• FRM-QA 4 Refrigeration Outfit Guide

Acceptable Specimen Type(s)

Serum

Test Request

• GPHL Apollo Web Portal
• Web Portal Instructions
• For manual orders, download the GPHL Submission Form

Possible Results

• Reactive
• Nonreactive
• Unsatisfactory

Interpretation of Hepatitis B Serologic Test Results

Reference Range

Nonreactive

Unacceptable Conditions

• Missing testing requisition
• Missing minimum of 2 patient identifiers
• Improper labeling
• Improper storage/transport of specimen
• No specimen received (empty collection tube).
• Wrong specimen type
• Low volume
• Specimen damaged, broken, or leaking upon arrival
• Specimen grossly hemolyzed, lipemic, turbid, or contaminated
• Plasma Samples
• Received refrigerated specimen over 7 days old

Interfering Substances

Hemolysis, lipemia, turbidity, microbial contamination, particulate matter or erythrocyte debris

Result Reported

3-5 business days

5-7 business days – Antigen positives

Fees

Fee Schedule

LOINC/Order Codes

77190-7 (Order code: 1410)