Special Bacteriology

Brief Description

The Bacteriology Unit accepts reference cultures from public and private health care providers for the identification, confirmation, and/or serotyping of bacterial isolates.  This includes a wide variety of aerobic, and facultative organisms isolated from clinical sources.  Isolates should be submitted as pure cultures.  Techniques used in the identification process include a combination of some or all the following: cellular and colonial morphology, conventional biochemical tests, Matrix Assisted Laser Desorption-Time of Flight (MALDI-TOF) mass spectrometry, PCR and 16S sequencing.

Laboratory Unit

Bacteriology

Methodology

  • Biochemicals
  • Serotyping
  • MALDI –TOF
  • Latex agglutination
  • PCR
  • 16S rDNA sequencing

Specimen Collection

Pure Isolate:

Collect in commercially available slant (Chocolate agar slant, blood agar slant, Tryptic soy agar slant).

Storage/Transport

Cultures can be maintained at room temperature (15-30°C) during transit as soon as possible after collection. 

Acceptable Specimen Type(s)

Pure Isolate

Test Request

Possible Results

  • Organisms are identified to the genus and species.
  • Non-viable culture
  • Unsatisfactory

Reference Range

N/A

Unacceptable Conditions

  • Missing testing requisition
  • Missing minimum of 2 patient identifiers
  • Improper labeling
  • Improper storage/transport of specimen
  • No specimen received (empty collection tube).
  • Wrong specimen type
  • Specimens damaged, broken, or leaking upon arrival non-viable cultures: all cultures received will be sub-cultured upon receipt. 
  • Cultures overgrown with contaminants
  • Specimens improperly collected or submitted

Interfering Substances

N/A

Result Reported

3-5 business days

Fees

Download this pdf file. Fee schedule

LOINC/Order Codes

6463-4 (Order code: 1130)