All isolates of STEC recovered from specimens by other clinical laboratories in Georgia should be referred to the Bacteriology Unit, either directly or through the Emerging Infections Program (EIP) site. Referred isolates will be further characterized by various methods, such as biochemical reactions, serogrouping and serotyping, Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF).
- Collect in the Para-Pak C&S outfit, orange-colored top (do not use the Parasitology O&P kit, blue and white-colored top, because it contains formalin that kills bacteria). Collect freshly passed feces as soon as possible after onset of illness and before antimicrobial therapy has been initiated. The patient should be cautioned against the use of antacid, barium, bismuth, anti-diarrheal medication, or oily laxatives prior to specimen collection. An appropriate (i.e., bloody, slimy, watery) area of stool should be selected and sampled with the collection spoon provided on the cap of the container. Add specimen only to the red line on the vial, tighten the cap securely, and invert several times. The solution in the vial should be salmon colored before the specimen is added. Three consecutive specimens collected on different days during the acute stage of diarrheal disease are recommended (first three days).
- Rectal swab:
- Place in a Culturette with Stuarts, Cary-Blair, or other commercially available transport medium.
- Culture Isolate and Broth:
- Submit an overnight, pure culture of the isolated bacteria on carbohydrate-free media available commercially (TSA, TSI and LIA are acceptable). GN broth and MAC broth are acceptable only for STEC.
- Feces can be maintained at refrigerated temperature (2-8°C) during transit if testing can be done within seven days of collection.
- Feces should be stored frozen (-20°C or colder) and shipped with dry ice after seven days of collection.
- Rectal swabs can be maintained at room temperature (15-30°C) during transit.
- Cultures can be maintained at room temperature (15-30°C) during transit.
Acceptable Specimen Type(s)
- Rectal swab
- Pure culture
- GPHL Apollo Web Portal
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- Identification to the genus and species
- Missing testing requisition
- Missing minimum of 2 patient identifiers
- Improper labeling
- Improper storage/transport of specimen
- No specimen received (empty collection tube).
- Wrong specimen type
- Specimens damaged, broken, or leaking upon arrival
- Specimens submitted in wrong preservative, e.g., PVA or 10% formalin
- Use of Parasitology outfit
- Multiple specimens collected on the same day
- Specimens received more than 4 days after collection (per Para-Pak C & S )
Use of antacid, barium, bismuth, anti-diarrheal medication, or oily laxatives.
4-6 business days
- 44089-1 (Culture Order code: 1070)
- 82303-9 (Clinical specimen Order code: 114006)