Syphilis-Routine RPR with Reflex to Anti-Treponemal Antibody
Brief Description
CDC recommendations are that all non-treponemal test be confirmed by a treponemal assay (traditional algorithm). Specimens will be screened by an RPR test that uses charcoal agglutination for detection of reagin antibodies and reflex all positive samples to a treponemal assay for confirmation.
Laboratory Unit
Immunology GPHL
Serology WPHL
Methodology
RPR charcoal agglutination
Chemiluminescence Immunoassay
Specimen Collection
Serum:
Collect in gold top SST tube, centrifuge.
Collect in tiger top SST tube, centrifuge.
Storage/Transport
Serum separated from the clot can be maintained at refrigerated temperature (2-8°C) during transit for no more than five days after collection.
Serum separated from the clot can be aliquoted, stored frozen (-20°C or colder) and shipped with dry ice.
Acceptable Specimen Type(s)
Serum
Test Request
- GPHL Apollo Web Portal
-
Web
Portal Instructions
- For manual orders, download the
GPHL
Submission Form
Possible Results
- Reactive
- Non-reactive
- Unsatisfactory
Reference Range
Non-reactive
Unacceptable Conditions
- Plasma Specimens
- Missing testing requisition
- Missing minimum of 2 patient identifiers
- Improper labeling
- Improper storage/transport of specimen
- No specimen received (empty collection tube).
- Wrong specimen type
- Specimen damaged, broken, or leaking upon arrival
- Grossly hemolyzed or lipemic
- Specimen containing microbial contamination
- Received more than 5 Days after collection
Interfering Substances
Hemolysis, lipemia, and bacterial contamination
Result Reported
5-7 business days
Fees
LOINC/Order Codes
20507-0 (Order code:1610 Decatur, W20000 Waycross)