Tetramine in Urine

Brief Description

This LRN-C developed method is for the quantitative determination of Tetramine in urine samples using gas chromatography with mass selective detection. The method uses Solid Phase Extraction (SPE) sample preparation and isotopically labeled internal standard for detection and quantification of Tetramine. 

Laboratory Unit

Chemical Threat



Specimen Collection


Spot Urine should be collected in a clean sterile urine container. The minimum volume for collection is 1.5 mL of urine. Urine should be frozen. Two empty containers of the same lot number should be submitted with the specimen.


Consultation is required with GPHL Emergency Response Coordinator Req 24/7 contact number: 866-782-4584.

If samples are being submitted from a known or suspected intentional chemical release, forensic integrity should be maintained. Two layers of forensic evidence tape should be applied. Initials of the person sealing the containers should appear half on and half off the tape.

Urine  should be stored frozen (-20°C or colder) and shipped with dry ice.

Acceptable Specimen Type(s)


Test Request

Possible Results

  • 5.0 µg/L -500 µg/L
  • Not detected
  • Unsatisfactory
  • PGC (Present but greater than highest calibrator)

Reference Range

Not detected

Unacceptable Conditions

  • Missing testing requisition
  • Missing minimum of 2 patient identifiers
  • No specimen received
  • Empty specimen container
  • Specimen leaking, damaged, or broken on arrival.
  • Insufficient specimen volume (less than 1.5 mL).
  • Suspected contamination
  • Clotting of the specimen
  • Expired sample collection container
  • Failure to maintain temperature
  • Frozen or hemolyzed specimens

Interfering Substances


Result Reported

1-3 business days

Turnaround times may be affected by the scale of the event.


No charge

LOINC/Order Codes

80649-7 (Order code: CT011300)