Bacillus anthracis Isolates

Brief Description

This real time Polymerase Chain Reaction (RT-PCR) molecular assay is intended for the qualitative detection of Bacillus anthracis DNA from culture isolates exhibiting colony morphology and bio-chemical testing consistent with an agent of bioterrorism. Additional reflex testing at the GPHL may be used to confirm specific agents.

Laboratory Unit

Molecular Biology



Specimen Collection

Culture isolates:

Submit a plate or slant exhibiting colony morphology and bio-chemical testing consistent with Bacillus anthracis on BAP agar, CHOC agar or TSA slant.


Consultation with district epidemiologist required prior to submission.

Cultures can be maintained at ambient temperature (15-30°C) during transit.

Acceptable Specimen Type(s)

Culture Isolates

Test Request

Possible Results

  • Detected
  • Not detected
  • Unsatisfactory
  • Inconclusive

Reference Range

Not detected

Unacceptable Conditions

  • Missing testing requisition form
  • Missing minimum of 2 patient identifiers
  • Improper labeling
  • Specimen damaged, broken, or leaked in transit
  • Improper storage/transport of specimen
  • No specimen received (empty collection tube).
  • Wrong specimen type
  • Low volume

Interfering Substances


Result Reported

1-2 business days

Turnaround times may be affected by the scale of the event.


No charge

LOINC/Order Codes

11469-4 (Order code: BTC01005)

Additional Information

This test will only be used in response to a biothreat event.  Requestor must contact GPHL at 1-866-PUB-HLTH (1-866-782-4584) prior to submitting specimens for testing.