This RT-PCR multiplex assay is intended for the qualitative detection of Bordetella pertussis, B. parapertussis, and B. holmesii in DNA extracted from clinical specimens or culture isolates. This assay may be used along with culture, other diagnostic assays, and clinical observations of signs or symptoms to determine infection in clinical specimens.
- Nasopharyngeal (NP) swab: (Culture and PCR)
- Submit Dacron swabs with aluminum shafts from a dry sterile container placed immediately into in a Regan-Lowe semi-solid agar or Amies charcoal gel transport tube.
- Nasopharyngeal (NP) swab: (PCR only)
- Submit Dacron swabs with aluminum shafts from a dry sterile container placed immediately into a dry, sterile tube or in a tube of liquid universal transport medium (UTM).
- Nasopharyngeal aspirates:
- Collect a minimum of 500µL in a sterile leak-proof plastic container of physiological saline.
- Culture isolates:
- Submit a plate or slant exhibiting colony morphology and biochemical testing consistent with Bordetella spp. on Regan-Lowe solid agar, Bordet Gengou solid agar, 5% sheep blood solid agar, Regan-Lowe semi-solid agar transport, or Amies charcoal gel transport.
Consultation with district epidemiologist required prior to submission.
- Nasopharyngeal swab (Culture and PCR) can be maintained at refrigerated temperature (2-8°C) within 30 minutes of collection and during transit for no more than 24-72 hours after collection.
- Nasopharyngeal swab (PCR only) should be stored frozen (-20°C or colder) within 30 minutes of collection and shipped with dry ice.
- Nasopharyngeal aspirates can be maintained at refrigerated temperature (2-8°C) within 30 minutes of collection and during transit for no more than 72 hours after collection. Nasopharyngeal aspirates can also be stored frozen (-20°C or colder) within 30 minutes of collection and shipped with dry ice.
- Cultures can be maintained at ambient temperature (15-30°C) or refrigerated temperature (2-8°C) during transit for no more than 24-48 hours after growth is present.
Acceptable Specimen Type(s)
- Nasopharyngeal (NP) swab
- Nasopharyngeal aspirates
- Culture isolates
- GPHL Apollo Web Portal
- For manual orders, download the
NOTE: A separate submission form is required for each specimen submitted for testing.
- Not Detected
- Missing testing requisition
- Missing minimum of 2 patient identifiers
- Improper labeling
- Improper storage/transport of specimen
- No specimen received (empty collection tube)/no growth on media plates or slants submitted for isolate testing
- Wrong specimen type
- Low volume (< 500 µL)
- Cotton swabs
1-3 business days
Turnaround times may be affected by the scale of the event.
23826-1 (Order code: 414000)