Burkholderia mallei/pseudomallei Isolates
Brief Description
This real time Polymerase Chain Reaction (RT-PCR) molecular assay is intended for the qualitative detection of Burkholderia mallei/pseudomallei DNA from culture isolates exhibiting colony morphology and bio-chemical testing consistent with an agent of bioterrorism. Additional reflex testing at the GPHL may be used to confirm specific agents.
Laboratory Unit
Molecular Biology
Methodology
RT-PCR
Specimen Collection
Culture isolates:
Submit a plate or slant exhibiting colony morphology and biochemical testing consistent with Burkholderia mallei/pseudomallei on BAP agar, CHOC agar or TSA slant.
Storage/Transport
Consultation with district epidemiologist required prior to submission.
Cultures can be maintained at ambient temperature (15-30°C) during transit.
Acceptable Specimen Type(s)
Culture Isolates
Test Request
- GPHL Apollo Web Portal
-
Web
Portal Instructions
- For manual orders, download the
GPHL
Submission Form
Possible Results
- Detected
- Not detected
- Unsatisfactory
- Inconclusive
Reference Range
Not detected
Unacceptable Conditions
- Missing testing requisition
- Missing minimum of 2 patient identifiers
- Improper labeling
- Improper storage/transport of specimen
- No specimen received (empty collection tube).
- Wrong specimen type
Interfering Substances
N/A
Result Reported
1-2 business days
Turnaround times may be affected by the scale of the event.
Fees
No charge
LOINC/Order Codes
44798-7 (Order code: BTC03005)
Additional Information
This test will only be used in response to a biothreat event. Requestor must contact GPHL at 1-866-PUB-HLTH (1-866-782-4584) prior to submitting specimens for testing.