Brucella spp.

Brief Description

This real time Polymerase Chain Reaction (RT-PCR) molecular assay is intended for the qualitative detection of Brucella spp. DNA from clinical specimens exhibiting colony morphology and bio-chemical testing consistent with an agent of bioterrorism. Additional reflex testing at the GPHL may be used to confirm specific agents.

Laboratory Unit

Molecular Biology



Specimen Collection

  • Whole blood:
    • Collect in a sterile EDTA tube.
  • Serum:
    • Collect in an SST tube, centrifuge.

NOTE: Samples below may only be submitted when coordinated with law enforcement.

  • Environmental samples (Swab, powder, liquid, envelope, letter, or package):
    • Collect in sterile container.


Consultation with district epidemiologist required prior to submission.

  • Whole Blood can be maintained at refrigerated temperature (2-8°C) during transit. 
  • Whole Blood can also be stored frozen (-20°C or colder). If previously frozen, ship with dry ice.
  • Serum can be maintained at refrigerated temperature (2-8°C) during transit.
  • Environmental samples can be maintained at ambient temperature (15-30°C) during transit.
  • Download this pdf file. FRM-QA 4 Refrigeration Outfit Guide

Acceptable Specimen Type(s)

  • Whole blood
  • Serum
  • Environmental samples

Test Request

Possible Results

  • Detected
  • Not detected
  • Unsatisfactory
  • Inconclusive

Reference Range

Not detected

Unacceptable Conditions

  • Missing testing requisition form
  • Missing minimum of 2 patient identifiers
  • Improper labeling
  • Specimen damaged, broken, or leaked in transit
  • Improper storage/transport of specimen
  • No specimen received (empty collection tube).

Interfering Substances


Result Reported

1-2 business days

Turnaround times may be affected by the scale of the event.


No charge

LOINC/Order Codes

552-0 (Order code: BTC02000)

Additional Information

This test will only be used in response to a biothreat event.  Requestor must contact GPHL at 1-866-PUB-HLTH (1-866-782-4584) prior to submitting specimens for testing.